Ionized calcium istat free#

Our study had several objectives over 2 experiments. To the best of our knowledge, only 1 study has evaluated the performance of the POC VS in bovine blood ( Moreover, results for a single test-cartridge take 1.5 min to output, adding to the time and cost of analysis. The average cost of the simplest POC VS cartridge that offers iCa measurement (CG8 +) is approximately $17.50, which substantially limits common use of this device in the field. Currently, 1 point-of-care ( POC) device that employs direct potentiometry ( POC VS VetScan i-STAT Abaxis North America, Union City, CA) is commonly used by in-hospital dairy clinicians, consultants, and researchers for assessing individual animal iCa status. Testing is usually done in clinical pathology laboratories using dedicated blood-gas instrumentation equipped with a Ca-specific ISE however, the logistical difficulty of getting field samples to a laboratory decreases the availability of routine iCa testing. The introduction of ion-selective electrode ( ISE) technology in clinical medicine has allowed for the direct measurements of iCa in blood, serum, and plasma. This novel ion-selective electrode Ca-module could become a rapid low-cost tool for assessing iCa cow-side, while qualitatively allowing classification of subclinical hypocalcemia on-farm. Sensitivity and specificity for the prototype and the POC VS under farm conditions at 3 potential subclinical hypocalcemia cut points were 100 and ≥93.5%, respectively. Results from the prototype and POC VS cow-side were 0.01 mmol/L higher and 0.05 mmol/L lower, respectively, compared with results from the laboratory RA on heparinized blood. With heparin-balanced samples under laboratory conditions, the prototype and POC VS measured an average 0.04 mmol/L higher and lower, respectively, compared with the RA. Ionized Ca results from the prototype cow-side were, on average, 0.06 mmol/L higher than the POC VS. A total of 101 periparturient Holstein cows from 3 dairy farms in New York State were used for the second experiment. Finally, sensitivity and specificity of the results obtained by the prototype and the POC VS cow-side compared with the results obtained by the laboratory RA using 3 different iCa cut points for classification of subclinical hypocalcemia were calculated. A follow-up validation experiment was performed, in which our objectives were to (1) assess the performance of the prototype cow-side against the POC VS (farm gold-standard) using fresh non-anticoagulated whole-blood samples (2) assess the performance of the prototype and the POC VS against the RA in a diagnostic laboratory using blood collected in a heparin-balanced syringe and (3) assess the agreement of the prototype and POC VS on-farm (fresh non-anticoagulated whole blood) against the RA on heparin-balanced blood. Coefficient of variation on 3 samples with high, within-range, and low iCa concentrations ranged from 1.0 to 3.9% for the prototype. A linearity experiment showed no deviation from linearity over a range of iCa concentrations compared with a commercial point-of-care (POC) device commonly used in the field (POC VS VetScan i-STAT, Abaxis North America, Union City, CA) and a laboratory gold standard benchtop blood-gas analyzer.

Ionized calcium istat free#

These flexible, easy-to-use analyzers help free your clinicians to focus on improved patient care without reliability or maintenance worries.The objective of this study was to assess an optimized ion-selective electrode Ca-module prototype as a potential cow-side device for ionized Ca (iCa) measurements in bovine blood. Designed to meet the challenges of point-of-care settings, RAPIDPoint ® 500 Blood Gas Systems leverage proven Siemens technology to deliver fast, accurate and comprehensive test results in approximately 60 seconds.